Screening of Aflatoxin Production by Aspergillus flavus Isolates from Petroleum-contaminated Soil

Fungi are eukaryotic, heterogeneous, unicellular to filamentous, spore-bearing, and chemoorganotrophic organisms which lack chlorophyll. This present study was carried out isolate identify fungi from petroleum-contaminated soil. Several fungal genera included Rhizopus spp., Mucor Penicillium Rhizoctonia Aspergillus Alternaria Cladosporiumspp. were isolated using potatoes dextrose agar, Czapek-Dox Agar, flavus Differentiation Agar culture media that comparable with co-amoxiclav (1g) chloramphenicol prevent the growth of any bacteria. The direct plate serial dilution agar methods used for isolation fungi. Based on results, Aspergillusand spp. most predominant had highest number colonies in soil samples. In this investigation, seven 27 samples morphologically (macroscopically microscopically) identified, such as A. flavus. Aflatoxigenicity detected characteristics differentiation colony fluorescence exposure ultraviolet light. Moreover, molecular approaches detection aflatoxigenic isolates. Three structural (aflD, aflO, aflP) one regulatory (aflR) gene aflatoxin cluster targeted amplification by polymerase chain reaction method. all six isolates molecularly contained two aflP)genes three genes, while there no specific aflO fourth, fifth, sixth issimilar aflR first second